![]() Growing high-quality protein crystals is a major bottleneck in crystal-based structure determination, which accounts for ∼90% of all structures in the Protein Data Bank (PDB) (Derewenda, 2011 ▸ Fazio et al., 2014 ▸ Lynch et al., 2020 ▸). Polo is free to use and modify for both academic and commercial use under the terms of the copyleft GNU General Public License v3.0. Crystallization images need not have been captured at HWI to utilize Polo’s basic functionality. Polo provides a streamlined one-click graphical interface for the Machine Recognition of Crystallization Outcomes (MARCO) convolutional neural network for automated image classification, as well as powerful tools to view and score crystallization images, to compare crystallization conditions, and to facilitate collaborative review of crystallization screening results. Polo aims to increase efficiency, reducing time spent manually reviewing crystallization images, and to improve the potential of identifying positive crystallization conditions. For example, you might keep images in a subfolder named Images (under the folder containing the Writer document) the recipient of the Calc file needs to put the images in a subfolder with the same name (under the folder containing the Writer document).Polo is a Python-based graphical user interface designed to streamline viewing and analysis of images to monitor crystal growth, with a specific target to enable users of the High-Throughput Crystallization Screening Center at Hauptman-Woodward Medical Research Institute (HWI) to efficiently inspect their crystallization experiments. You need to keep track of the location of the images and make sure the recipient knows where to put them on another machine, so the Calc document can find them. Disadvantage – If you send the document to someone else, or move it to a different computer, you must also send the image files, or the receiver will not be able to see the linked images.This can be a big advantage if you (or someone else, perhaps, a graphic artist) is updating images. Advantage – You can modify the image file separately without changing the document because the link to the file remains valid, and the modified image will appear when you next open the document.The file size is usually not a problem on a modern computer with a reasonable amount of memory, unless the document includes many large graphics files. Advantage – Linking can reduce the size of the document when it is saved, because the image file itself is not included.Linking an image has two advantages and one disadvantage: The document and the image remain as two separate files, and they are merged temporarily only when you open the document again. ![]() The image is then displayed in the document, but when the document is saved, it contains only a reference to the image file¿not the image itself. To create a link to the file containing the image instead of saving a copy of the image in the Calc document, use the Insert picture dialog and select the Link option. This method always embeds (saves a copy of) the image file in the Calc document. The picture will be anchored to the cell where it was dropped. A faint vertical line marks where the image will be dropped.
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